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fluo-4 calcium imaging kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fluo-4 calcium imaging kit
    Fluo 4 Calcium Imaging Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluo+4+calcium+imaging+kit/fluo+4+am/pm40652512-192-231-236
    Average 90 stars, based on 1 article reviews
    fluo-4 calcium imaging kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Ca v 3.2 T-type voltage-gated calcium channels and Ca 2+ influx
    Article Snippet: 300,000 cells were left to adhere overnight and cultured in Dulbecco's Modified Eagle's Medium (DMEM; Cellgro) supplemented with heat-inactivated foetal bovine serum (FBS, final concentration 10%; Atlanta Biologicals). .. Cells were exposed to either HCCMF (hepatocellular carcinoma specific AM RF EMF) or no treatment for 30 min, 1 h, 3 h, or 6 h. 30 min before completion of treatment Fluo-4 stain mix was added directly to each well/dish (Fluo-4 Calcium Imaging Kit; Molecular Probes). ..

    Article Title: Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Ca v 3.2 T-type voltage-gated calcium channels and Ca 2+ influx
    Article Snippet: .. All fluorescent data was read using a Fluostar fluorescent plate reader (unless otherwise stated i.e. flow cytometry) with 485 excitation/520 emission and calcium staining was accomplished using the Fluo-4 calcium imaging kit (Molecular Probes). ..

    Imaging:

    Article Title: Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Ca v 3.2 T-type voltage-gated calcium channels and Ca 2+ influx
    Article Snippet: 300,000 cells were left to adhere overnight and cultured in Dulbecco's Modified Eagle's Medium (DMEM; Cellgro) supplemented with heat-inactivated foetal bovine serum (FBS, final concentration 10%; Atlanta Biologicals). .. Cells were exposed to either HCCMF (hepatocellular carcinoma specific AM RF EMF) or no treatment for 30 min, 1 h, 3 h, or 6 h. 30 min before completion of treatment Fluo-4 stain mix was added directly to each well/dish (Fluo-4 Calcium Imaging Kit; Molecular Probes). ..

    Article Title: IPSC-derived midbrain astrocytes from Parkinson’s disease patients carrying pathogenic SNCA mutations exhibit alpha-synuclein aggregation, mitochondrial fragmentation and excess calcium release
    Article Snippet: .. The Fluo-4 Calcium Imaging Kit (Invitrogen™, F10489) was used for the detection of cytosolic Ca 2+ according to the manufacturer’s guidelines. ..

    Article Title: Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes.
    Article Snippet: .. Fluo-4 Calcium Imaging Kit (Invitrogen F10489) was used to monitor calcium waves following manufacturer’s instructions. .. Calcium waves were captured using a Zeiss Observer Microscope.

    Article Title: Crosstalk Between Kcnk3-Mediated Ion Current and Adrenergic Signaling Regulates Adipose Thermogenesis and Obesity
    Article Snippet: .. The attached cells were loaded with Fluo-4 AM dye per manufacturer’s protocol (Fluo-4 Calcium Imaging Kit, Thermo Fischer Scientific {"type":"entrez-nucleotide","attrs":{"text":"F10489","term_id":"683147","term_text":"F10489"}} F10489 ) and imaged in HBSS with Ca 2+ & Mg 2+ (Corning 21-023-CV) + 20 mM Glucose. .. Images were acquired every 4 s with the Inverted Nikon Ti Fluorescence Microscope with a Plan Fluor 10x air immersion objective and data acquisition software MetaMorph.

    Article Title: Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Ca v 3.2 T-type voltage-gated calcium channels and Ca 2+ influx
    Article Snippet: .. All fluorescent data was read using a Fluostar fluorescent plate reader (unless otherwise stated i.e. flow cytometry) with 485 excitation/520 emission and calcium staining was accomplished using the Fluo-4 calcium imaging kit (Molecular Probes). ..

    Flow Cytometry:

    Article Title: Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Ca v 3.2 T-type voltage-gated calcium channels and Ca 2+ influx
    Article Snippet: .. All fluorescent data was read using a Fluostar fluorescent plate reader (unless otherwise stated i.e. flow cytometry) with 485 excitation/520 emission and calcium staining was accomplished using the Fluo-4 calcium imaging kit (Molecular Probes). ..



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